Cytotoxicity

Guava Cell Toxicity Assay

Simple and superior

Cytotoxicity determination has never been easier to assess than with the Guava® Cell Toxicity Assay.

This single-cell based assay provides results that are as good as the 51-Chromium release assay, but without radioactivity. As a result, scientists don't need to worry about potential hazards and disposal of radioactive material.

What's more, the assay provides results that are more accurate and precise than population-based methods such as alamarBlue, MTT, and LDH.

CMC, CDC, ADCC, NK

All of the common methods used to study cytotoxicity can be performed using the Guava Cell Toxicity Assay, including cell-mediated or cell-dependent cytotoxicity (CMC or CDC), antibody-dependent cell mediated cytotoxicity (ADCC), and natural killer cytotoxicity (NK). The Guava Cell Toxicity Assay provides all relevant statistics, including percentage of target cells killed, effector and target cell percentages, and whether the cells are alive or dead.

Sensitive and reproducible

The assay uses a well-characterized cell tracking dye that is optimized for use on all Guava Systems. The dye diffuses freely into cells and is retained within the cell without affecting cellular function. Because the assay is both sensitive and reproducible, assay development time is reduced.

Key features

• Tube and plate-based protocols
• Works with adherent and suspended cells
• Staining and data acquisiton complete in about 4 hours
• Provides direct, absolute cell counts and population percentages
  
• Analyzes cytotoxicity in single cells, discerning target cells from effector cells and provides all relevant statistics including % Target Cells Killed

Key references

Pattijn S, Fishwild D, Lem L, Van der Aa, A. Alternative non-radioactive assay to measure antigen-specific cytotoxic T cells: A head-to-head comparison of the Guava® Cell Toxicity Assay with the traditional 51Cr release assay. Innogenetics NV, Guava Technologies, Inc. Presented at MASIR, Santorini, Greece, June 14-17, 2006.

Van der Aa, A. Measuring cytotoxic properties of antigen-specific T cells using cytometry: The non-radioactive Guava® Cell Toxicity Assay as an alternative to the 51Cr release assay. R&D Therapeutics, Immune Response Unit, Cytometry, Bruges, October 19, 2006.

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