Cell Tracking

Guava Cell Paint Assay
for tracking target cells in mixed populations

The Guava® Cell Paint Assay offers single-cell analysis capabilities for mixed cultures without the complexity of traditional methods. The assay tracks one cell population from a second in mixed populations with the sensitivity of single-cell based methods and the throughput of a microplate format.

Easier than traditional methods

Using a well-characterized fluorescent dye, carboxyfluorescein diacetate succinimidyl ester (commonly referred to as CFSE), that diffuses freely into cells and is retained within the cell without affecting cellular function, The Guava Cell Paint Assay easily differentiates among cell populations based on the single dye or user-defined dual-color options. The assay system is easier to use than traditional methods, and because it is optimized for Guava Systems, assay development time is significantly reduced, increasing laboratory productivity.

Second dye can be added to monitor antigen specificity or protein expression

While the assay uses the cell tracking dye only as a single-color assay to track target cells within a mixed population for in vivo and in vitro applications, a second dye can be added to perform a user-defined dual-color assay for monitoring protein expression or antigen specificity. The two-color mode is particularly useful for monitoring specificity of mixed cultures in the same tube, say to determine antigen specificity in mixed cultures in a single tube or well, thus reducing the number of tubes or wells required to run the assay.

Key features include

• Tube- and plate-based protocol
  
• Works with adherent and suspended cells
• Analyzes single cells
• Provides direct, absolute cell counts and population percentages
• Data compatible with third party analysis software
  
• Less expensive alternative to conjugated antibodies
• Staining and data acquisition in <3 hours
• Runs on Guava PCA, PCA-96, EasyCyte Mini and EasyCyte Plus Systems
  
• Applicable to many mixed culture assays:
• Specificity of hybridoma supernatants
• Protein expression
• Apoptosis induction
• Co-cultures (feeder layers)

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