Cell Proliferation

Guava Cell Growth Assay
for assessment of cell proliferation in primary cultures

Assessments out to 5 generations

The Guava® Cell Growth Assay provides, at the single-cell level, proliferation assessments out to five generations with a simple, non-radioactive assay that is superior to population-based methods such as 3-Thymidine and MTT (or XTT). In addition, the assay offers a broader linear range when compared to colorimetric methods.

Simple, non-radioactive protocol 

The assay uses a well-characterized fluorescent dye, carboxyfluorescein diacetate succinimidyl ester (commonly referred to as CFSE), that diffuses freely into cells and is retained within the cell without affecting cellular function. The dye is not transferred to adjacent cells. For each round of cell division, the relative fluorescence intensity of the dye is decreased by half. A dead cell dye, PI, is included to discern the percentage of cells that are dead.

The assay is ideal for primary cultures such as peripheral blood mononuclear cells (PBMCs), activated T- or B-cells, peripheral blood lymphocytes (PBLs), thymocytes, hematopoietic cells, and splenocytes.

The assay provides a powerful tool with which to assess cell doubling time, verify the health of cell cultures, or screen for compounds that affect cell growth

Key features include

• Microplate-based protocol
• Analyzes division and proliferation in single primary cells
• Works with adherent or suspended cells: PMBCs, PBLs, thymocytes, hematopoietic cells, splenocytes, activated T- or B-cells
• Staining and data acquisition complete in about 7 hours
• Distinguishes proliferated or resting, live or dead cells
• Provides direct, absolute cell counts and population percentages
• Data compatible with third party analysis software
• Runs on Guava EasyCyte Plus System
  

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