Mitochondrial Depolarization

Guava Mitochondrial Depolarization Assay
for detection of early apoptosis

There is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility.

Microplate protocol

Guava Technologies has optimized an assay format for 96-well plates that combines the sensitivity of a single-cell based method with the throughput and ease-of-use of a microplate reader. The Guava® Mitochondrial Depolarization Assay for the Guava EasyCyte™ Plus System allows you to monitor the loss of mitochondrial membrane potential through the use of the fluorescent cationic dye, JC-1. An optional dead cell dye, 7-AAD, is also included for discerning apoptotic cells from dead cells.

Distinguishes healthy, early apoptotic, and dead cells

In healthy, non-apoptotic cells, the JC-1 dye enters the mitochondrial matrix, accumulates as aggregates and stains the mitochondria bright red. In apoptotic cells, the mitochondrial membrane potential collapses, and the JC-1 enters the cytoplasm as a monomeric form where it fluoresces green. Results are automatically calculated and presented in a statistical format that is easy to interpret, with total cell counts and percentages for those cell populations that are either healthy, apoptotic, or dead.

Key features include

• Mix-and-read, tube or 96-well plate protocol
  
• Analysis of membrane potential in single cells
• Works with adherent or suspended cells
• Staining and data acquisition in ≤1 hours
• Provides direct, absolute cell counts and populations percentages
• Data compatible with third party analysis software
• Runs on Guava EasyCyte Mini and Plus Systems
  

Ordering information