Guava Caspase 3/7 and 8 Assays
for detection of mid-stage apoptosis
The Guava® Caspase 3/7 and 8 Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspases) reagent that specifically identifies active caspase 3 and 7 molecules or caspase 8 molecules.
Non-cytotoxic, cell permeant dyes elimiate lysis and permeabilization procedures
Each FLICA is comprised of three subunits: A caspase-specific amino acid inhibitor containing a recognition sequence for caspase 3/7 (DEVD) or caspase 8 (LETD); a fluoromethylketone moiety (FMK) that forms a covalent linkage with the active enzyme; and a carboxyfluorescein (FAM) reporter. The nuclear DNA stain is also included in the assay to simultaneously evaluate membrane integrity and cell viability. The assay detects three populations:
Healthy or early apoptotic cells
mid-apoptotic cells
late apoptotic or dead cells
Key features include
Tube and 96-well plate protocol
Works with adherent and suspended cells
Analyzes apoptosis in single cells
FLICA reagents are non-cytotoxic and cell permeant, thus eliminating cell lysis and permeabilization procedures
Staining and data acquisition in ≤3 hours
Provides direct, absolute cell counts and population percentages
Data compatible with third party analysis software
