Antibody Quantification

Guava RapidQuant Assays
for quantificatoin of secreted IgG antibodies

Quick and easy

With its simplicity and ease of use, the Guava RapidQuant™ Assays have raised the bar for IgG titering. Designed to be time-saving, labor-saving and money-saving, RapidQuant Assays eliminate the most tedious, laborious portions of antibody titering methods.

Sensitive and reliable

RapidQuant Assays are bead-based and rely on fluorescence for the quantitative measurements. The fluorescent intensity is proportional to the concentrations of antibody captured on the beads. Using fluorescence provides tremendous reliability and precision so you can have confidence in every result. 

Highly specific

RapidQuant Assays have been tested extensively with both human and mouse antibodies of all IgG subtypes and in many types of hybridoma media and have performed well in all cases tested. RapidQuant IgG Kits are extremely specific and will not be hindered by the presence of FBS or IgM in the media. The assay can be used to determine relative antibody production levels by different hybridoma clones or to determine absolute concentrations of antibody by comparison to a standard curve.

Unprecedented stability

RapidQuant Kits also require very small volumes of sample for accurate determinations of antibody concentation so you can perform multiple analyses of each supernatant from a single 96-well plate.
Unlike many current colorimetric immunoassays, the fluorescence readout allows multiple plates to be prepared, refrigerated and read hours or days later, without a loss of signal. This unprecedented stability and robustness makes the assay ideal for large scale screening on robotic systems where multiple plates are prepared prior to sampling.

Key features include

• Microplate-based protocol
• Works with hybridoma supernatant
• Staining and data acquisition in <2 hours
• Results include standard curve and IgG concentration
  

Available reagent kits

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